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Product Details
Synonyms
CD117, SCFR, c-Kit, KIT
Source
Human CD117, His Tag (CD7-H52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Thr 516 (Accession # P10721-2).
Predicted N-terminus: Gln 26
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 57.1 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human CD117, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human CD117, His Tag (Cat. No. CD7-H52H4) is more than 90% and the molecular weight of this protein is around 75-100 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human CD117, His Tag (Cat. No. CD7-H52H4) at 2 μg/mL (100 μL/well) can bind Biotinylated Human SCF, Avitag,His Tag (Cat. No. SCF-H82E1) with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human SCF, Avitag,His Tag (Cat. No. SCF-H82E1) captured on Biotin CAP-Series S Sensor Chip can bind Human CD117, His Tag (Cat. No. CD7-H52H4) with an affinity constant of 0.236 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
V***l2022-06-25
LikesWe used the c-Kit protein to immunizations projects we are carrying for our studies and they performed great. The delivery was also very fast and the purchasing process very easy. We also purchased several other antibodies and recombinant proteins that we are currently testing and they all have very good quality. I have not used directly the technical services because it was not necessary, as the website is very easy and informative, so I found the products we wanted with no problem.
Background
Recent Advances
Drug Development Progress- English Name：
Stem cell growth factor receptor
- Category：
- Approved Drugs：
17 Details
- Drugs in Clinical Trials：
40 Details
- Highest Development Stage：
Approved
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