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Product Details
Synonyms
APRIL, TNFSF13, TALL-2, TRDL-1, CD256, TALL2, ZTNF2
Source
Biotinylated Cynomolgus APRIL Protein, Avitag,His Tag (APL-C82Q3) is expressed from human 293 cells (HEK293). It contains AA Gln 111 - Leu 250 (Accession # XP_005582818.1).
Predicted N-terminus: Gly
Molecular Characterization
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 50.9 kDa. The protein migrates as 55-65 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus APRIL Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus APRIL Protein, Avitag,His Tag (Cat. No. APL-C82Q3) is more than 95% and the molecular weight of this protein is around 50-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Cynomolgus / Rhesus macaque BCMA, Fc Tag (Cat. No. BCA-C5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus APRIL Protein, Avitag,His Tag (Cat. No. APL-C82Q3) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Immobilized Human TACI (R119A, R122A), Fc Tag (Cat. No. TAI-H5253) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus APRIL Protein, Avitag,His Tag (Cat. No. APL-C82Q3) with a linear range of 0.2-5 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Cynomolgus APRIL Protein, Avitag,His Tag(Cat. No. APL-C82Q3) immobilized on SA Chip can bind Cynomolgus / Rhesus macaque BCMA Protein, Fc Tag (Cat. No. BCA-C5253) with an affinity constant of 0.183 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 13
- Category：
- Approved Drugs：
2 Details
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30 Details
- Highest Development Stage：
Approved
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