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Product Details
Synonyms
4-1BB Ligand, TNFSF9, CD137L
Source
Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (41L-H52D4) is expressed from human 293 cells (HEK293). It contains AA Arg 71 - Glu 254 (Accession # P41273-1).
Predicted N-terminus: His
Molecular Characterization
This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.
The protein has a calculated MW of 62.8 kDa. The protein migrates as 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human 4-1BB Ligand (71-254) Protein, His,Flag Tag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) at 2 μg/mL (100 μL/well) can bind Human 4-1BB, Fc Tag (Cat. No. 41B-H5258) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Mouse 4-1BB, Fc Tag (Cat. No. 41B-M5258) on Protein A Biosensor, can bind Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) with an affinity constant of 18.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Human 4-1BB Ligand (71-254) Protein, His,Flag Tag (Cat. No. 41L-H52D4) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.20-0.44 μg/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 9
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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