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Product Details
Product Overview
Human Perforin ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human Perforin that is present in biological samples, such as human serum, plasma, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Standards to calibrate with NIBSC/WHO standards for comparable results.
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalytePerforinFormat96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity60 pg/mLAssay Time2.75 hrSample volume100 μLRange62.5 pg/mL-2000 pg/mLSample TypeCell Culture Supernatants, Plasma, Serum.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human Perforin in serum, plasma and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Materials Provided
IDComponentsSizeCEA034-C01Pre-coated Anti-Perforin Antibody Microplate1 plateCEA034-C02Human Perforin Standard1ng×2CEA034-C03Biotin-Anti-Perforin Antibody Con. Solution150 μLCEA034-C04Biotin-Antibody Dilution Buffer15 mLCEA034-C05Streptavidin-HRP Con. Solution1.5 mLCEA034-C06Streptavidin-HRP Dilution Buffer15 mLCEA034-C0720×Washing Buffer50 mLCEA034-C08Sample Dilution Buffer15 mL×2CEA034-C09Substrate Solution12 mLCEA034-C10Stop Solution6 mLACRO Quality Management System
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of Perforin is less than 60 pg/mL.
Intra-Assay Statistics
Ten replicates of each of three samples containing different Perforin concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of Perforin were tested in independent assays. Acceptable criteria: CV<15%.
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