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Product Details
Product Overview
Human FGF-21 ELISA Kit is a ready-to-use immunoassay kit, specifically designed to quantitate natural and recombinant human FGF-21 that is present in biological samples, such as serum, and cell culture supernatants. Our ELISA Kit provides several benefits:
- Fully validation in biologic samples for detection range, sensitivity, inter- and intra-plate CV, recovery, dilution linearity, specificity, and matrix effects to ensure reliable results according to ICH M10 guideline.
- High-quality antibody pairs and protein standards, along with rigorous quality control, to guarantee consistent results across different batches.
- Simplified and straightforward protocols and ready-to-use reagents to save assay time.
Product Specifications
Assay TypeSandwich-ELISAAnalyteFGF-21Format96-wells plate breakable into 12 x 8 wells stripsReactivityHumanSensitivity20 pg/mLAssay Time2.75 hrSample volume50 μLRange31.25 pg/mL-2000 pg/mLSample TypeFor the quantitative determination of Human FGF-21 in serum, plasma and cell supernatant.Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Application
The kit is developed for quantitative detection of natural and recombinant human FGF-21 in serum, and cell culture supernatants.
It is for research use only.
Workflow
Storage
Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of FGF-21 is less than 20 pg/mL.
Sample Values
Healthy donor serum samples (n=77) were evaluated for the concentrations of human FGF-21 in the assay. The detectability rate of the samples was 96%, with a mean detectable concentration of 282 pg/mL and a concentration range of 26.8 to 918.2 pg/mL.
Intra-Assay Statistics
Ten replicates of each of 3 samples containing different FGF-21 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Inter-Assay Statistics
Three samples containing different concentrations of FGF-21 were tested in independent assays. Acceptable criteria: CV<15%.
Recovery
Recombinant FGF-21 was spiked into 3 human serum samples, and then analyzed. The average recovery of FGF-21 for serum samples is 96.7%.
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Drug Development Progress- English Name：
Fibroblast growth factor 21
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
10 Details
- Highest Development Stage：
Phase 3 Clinical
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