Product Details
Source
Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
14E1
Isotype
Human IgG1, Kappa
Host Species
Mouse
Reactivity
Virus
Immunogen
Recombinant Coxsackievirus A16 (strain G-10) VP0 Protein is expressed from human 293 cells
Specificity
Specifically recognizes Coxsackievirus A16 (strain G-10) VP0 Protein.
Application
ApplicationRecommended UsageWestern Blot10-0.02 μg/mLCross Verification
The antibody can be used for WB detection.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA

Immobilized Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag (Cat. No. VP0-C52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) (Cat. No. VP0-MY2108) with a linear range of 0.1-4 ng/mL (QC tested).
Protocol
Western Blot

Detection of Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) by Western Blot. Monoclonal Anti-Coxsackievirus A16 (strain G-10) VP0 Antibody, Human IgG1 (14E1) at 0.02 μg/mL dilution + Coxsackievirus A16 (strain G-10) VP0 Protein, His Tag at 400 ng.<br>
Secondary: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
Predicted band size: 40-60 kDa 12% Bis-Tris Protein Gel.
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