Product Details
Source
Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.
Antibody Type
Recombinant Monoclonal
Clone
7E12
Isotype
Human IgG1, Kappa
Host Species
Mouse
Specificity
This product is a specific antibody specifically reacts with NS1.
Application
ApplicationRecommended UsageWestern Blot0.1-10 ug/mLPurification
Protein A purified / Protein G purified.
Aggregation
Less than 10%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA

Immobilized DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) (Cat. No. DE1-M756) with a linear range of 0.06-2 ng/mL (QC tested).
Protocol
Bioactivity-SPR

Monoclonal Anti-DENV 2(strain Thailand/16681/1984)-NS1 Antibody, Human IgG1 (7E12) (Cat. No. DE1-M756) captured on Protein A Chip can bind DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (Cat. No. NS1-D52H3) with an affinity constant of 0.167 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Western Blot

Detection of Anti-DENV 2(strain Thailand/16681/1984)-NS1-7E12,Human,Human IgG1 | Human Kappa,HEK by Western Blot. Anti-DENV 2(strain Thailand/16681/1984)-NS1-7E12,Human,Human IgG1 | Human Kappa,HEK at 1/5000 dilution + DENV2 (strain Thailand/16681/1984) NS1 protein, His Tag (MALS verified) at 200ng.<br>
Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
Predicted band size: 45~55 kDa 12% Bis-Tris Protein Gel.
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