PE-Labeled Mouse IgG2a Antibody Isotype Control

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Cat. No. / Size
Price
Qty
DNP-PM487-25tests
$45.00
DNP-PM487-200tests
$205.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Mouse IgG2a Antibody Isotype Control is a monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Immunogen

    DNP (Dinitrophenyl)

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Specificity

    Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins, which is normally not present in Humans or animals. Therefore, DNP-PM487 does not react with any antigen of Human or animal cells.

  • Application

    Flow Cytometry.

  • Recommended Dilution

    1:50

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4) containing 0.2% BSA, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-FACS

     IgG control FACS

    Flow cytometric analysis of Anti-CD19 CAR-293 cells staining with PE-Labeled Mouse IgG2a Antibody Isotype Control (Cat. No. DNP-PM487) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with positive control antibody. PE signal was used to evaluate the binding activity (QC tested).

    Protocol

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Background

Fluorescently or biotin labeled isotype controls are used to assess non specific staining and autofluorescence in flow cytometry, ELISA, and imaging assays. By recognizing DNP, a hapten absent in biological samples, these controls provide a reliable baseline to distinguish background fluorescence or signal from true antigen specific binding.

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