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Human IgG1 Kappa Isotype Control (mAb, carrier free, MALS verified)

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Cat. No. / Size
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DNP-M2-100ug
$165.00
DNP-M2-1mg
$720.00
DNP-M2-25mg
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DNP-M2-100mg
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ETA of in-stock products:2 business days
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Product Details

  • Source

    Human IgG1 Kappa Isotype Control (mAb) is a recombinant chimeric monoclonal antibody expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Human

  • Specificity

    This product is a specific antibody specifically reacts with DNP.

  • Application

    Application
    Recommended Usage
    ELISA
    1:800-1:8000
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 5%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Sterility

    The sterility testing was performed by membrane filtration method.

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

    25 mg or larger size will be supplied as liquid and shipped by dry ice. Please inquire the dry ice shipping cost.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     IgG control ELISA

    Immobilized DNP-Albumin Conjugate at 10 μg/mL (100 μL/well) can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with a linear range of 1-10 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     IgG control SPR

    Human FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (Cat. No. FCM-H5286) captured on CM5 chip via anti-His antibody can bind Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) with an affinity constant of 97.8 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

Customer Reviews
Writing Reviews

  • S***r2022-10-19
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    Service with Acro and their customer support is as quick as they are helpful. Products ordered arrived in a timely manner. Customer support personal are as friendly as they are insightful. Never have they disappointed when purchasing any of their products. Such a user friendly website and if anything is not found customer support never backs down to help out!

Background

Isotype controls are essential to distinguish non specific binding from true antigen recognition. This antibody specifically recognizes DNP, a hapten not present in biological samples, making it an ideal negative control with minimal background in flow cytometry, ELISA, SPR/BLI, and cell based assays.

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  • Performance Data
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