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Product Details
Source
Biotinylated Mouse IgG1 Isotype Control (mAb) is a mouse monoclonal antibody recombinantly expressed in HEK293 cells.
Antibody Type
Recombinant Monoclonal
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Conjugate
Biotin
Specificity
Specifically reacts with DNP (Dinitrophenyl) and DNP conjugated proteins.
Purification
Protein A purified / Protein G purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Immobilized DNP-Albumin Conjugate, Bovine at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) with a linear range of 1-39 ng/mL (QC tested).
Protocol
Bioactivity-FACS
2e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 1 μg/mL of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1) and positive control respectively, washed and then followed by PE-SA and analyzed with FACS (QC tested).
Protocol
2e5 of Anti-CD19 CAR-293 cells were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
5e5 of PBMCs were stained with a series of concentrations of Biotinylated Mouse IgG1 Isotype Control (mAb) (Cat. No. DNP-BM1), washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Protocol
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