Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5)

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Cat. No. / Size
Price
Qty
HA2-Y290-100ug
$360.00
HA2-Y290-1mg
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) is a mouse monoclonal antibody derived from hybridoma cells fused with SP2/0 myeloma cells and murine B lymphocytes.

  • Antibody Type

    Hybridoma Monoclonal

  • Clone

    13A5

  • Isotype

    Mouse IgG1, Kappa

  • Host Species

    Mouse

  • Specificity

    This product is a specific antibody specifically reacts with HA.

  • Cross Verification

    This product can cross in Elisa with
    Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
    Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6).
    Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
    This product No cross-reactivity in ELISA with
    Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
    Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
    Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
    Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4).
    Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
    Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No.HA6-V52H3).
    Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
    Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
    Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7).
    Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
    Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
    Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
    Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
    Influenza A [A/Hong Kong/483/97 (H5N1)] HA, His Tag (Cat. No. HA1-V5229).

  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Hemagglutinin/HA (Influenza Virus) ELISA

    Immobilized Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.1-6 ng/mL (QC tested).

    Protocol
  •  Hemagglutinin/HA (Influenza Virus) ELISA

    Immobilized Influenza A [A/Darwin/9/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H6) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.2-13 ng/mL (Routinely tested).

    Protocol
  •  Hemagglutinin/HA (Influenza Virus) ELISA

    Immobilized Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.1-6 ng/mL (Routinely tested).

    Protocol
  •  Hemagglutinin/HA (Influenza Virus) ELISA

    Immobilized Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4)/Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with a linear range of 0.2-13 ng/mL (Routinely tested).

    Protocol
  • Bioactivity-SPR

     Hemagglutinin/HA (Influenza Virus) SPR

    Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5) captured on CM5 chip via anti-His antibody can bind Monoclonal Anti-HA-H3N2(Influenza A/Darwin/6/2021) Antibody, Mouse IgG1 (13A5) (Cat. No. HA2-Y290) with an affinity constant of 3.43 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol

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Background

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

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