Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (MALS verified)

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Cat. No. / Size
Price
Qty
HA1-M757-100ug
$360.00
HA1-M757-1mg
$2380.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) is a chimeric monoclonal antibody recombinantly expressed in HEK293, which combines the variable region of a mouse monoclonal antibody with human constant domain.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    1C10

  • Isotype

    Human IgG1, Kappa

  • Host Species

    Mouse

  • Specificity

    This product is a specific antibody specifically reacts with HA.

  • Application

    Application
    Recommended Usage
    Western Blot
    1-10 ug/mL
  • Cross Verification

    This product can cross in Elisa with
    Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H8).
    Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H7).
    Influenza A (A/Georgia/12/2022) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H7).
    This product No cross-reactivity in ELISA with Influenza A [A/Victoria/2570/2019] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H6).
    Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
    Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
    Influenza A [A/Darwin/6/2021 (H3N2)] HA Protein, His Tag (Cat. No. HA2-V52H5).
    Influenza A (Vietnam/1194/2004(H5N1)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA1-V52H9).
    Influenza A (Guangdong/18SF020(H5N6)) Hemagglutinin (HA) Protein, His Tag (Cat. No.HA6-V52H3).
    Influenza A (turkey/Germany-MV/R2472/2014(H5N8)) HA Protein, His Tag (Cat. No. HA8-V52H3).
    Influenza A (A/Shanghai/02/2013(H7N9)) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA9-V52H3).
    Influenza A [A/guinea fowl/Hong Kong/WF10/99(H9N2)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H7).
    Influenza B [Austria/1359417/2021 (B/Victoria lineage)] Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H3).
    Influenza B [Phuket/3073/2013 (B/Yamagata lineage)] HA Protein, His Tag (Cat. No. HAE-V52H4).
    Influenza A [A/Bangkok/1/1979 (H3N2)] HA, His Tag (Cat. No. HA2-V52H3).
    Influenza A [A/Wisconsin/588/2019 (H1N1)] HA, His Tag (Cat. No. HA1-V52H3).
    Influenza A Virus HA (H3N2) Protein, His Tag (Cat. No. H32-V52H3).
    Influenza B (B/Singapore/INFTT-16-0610/2016) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H5).
    Influenza B (B/Singapore/WUH4618/2021) Hemagglutinin (HA) Protein, His Tag (Cat. No. HAE-V52H6).
    Influenza A (A/Sydney/1304/2022) Hemagglutinin (HA) Protein, His Tag (Cat. No. HA2-V52H9).
    Influenza A Virus (A/District of Columbia/27/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H5).
    Influenza A Virus (A/Croatia/10136RV/2023) HA (H3N2) Protein, His Tag (Cat. No. H32-V52H4).

  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Hemagglutinin/HA (Influenza Virus) ELISA

    Immobilized Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-HA-H1N1(Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) with a linear range of 0.05-2 ng/mL (QC tested).

    Protocol
  • Bioactivity-SPR

     Hemagglutinin/HA (Influenza Virus) SPR

    Monoclonal Anti-HA-H1N1 (Influenza A/Sydney/5/2021) Antibody, Human IgG1 (1C10) (Cat. No. HA1-M757) captured on Protein A Chip can bind Influenza A [Sydney/5/2021 (H1N1)] HA Protein, His Tag (Cat. No. HA1-V52H4) with an affinity constant of 0.334 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

    Protocol
  • Western Blot

     Hemagglutinin/HA (Influenza Virus) WESTERN BLOT
  • Detection of Anti-HA-H1N1(Influenza ASydney52021)-1C10,Human,Human IgG1 Human Kappa by Western Blot. Anti-HA-H1N1(Influenza ASydney52021)-1C10,Human,Human IgG1 Human Kappa at 1/1000 dilution + Influenza A [Victoria/4897/2022] Hemagglutinin (HA) Protein, His Tag (MALS verified) at 200ng & Influenza A [Wisconsin/67/2022] Hemagglutinin (HA) Protein, His Tag (MALS verified) at 200ng & Influenza A [Sydney/5/2021 (H1N1)] Hemagglutinin (HA) Protein, His Tag (MALS verified) at 200ng.<br>
    Secondary Antibody: (HFC)-HRP Goat Anti-Human IgG,Fcγ fragment specific (min X Bov,Hrs,Ms Sr Prot) at 1/2000 dilution.<br>
    Predicted band size: 95 kDa&100 kDa&100 kDa 12% Bis-Tris Protein Gel.

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Background

Neuraminidase (NA) and hemagglutinin (HA) are major membrane glycoproteins found on the surface of influenza virus. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle. Hemagglutinin also plays a major role in the determination of host range restriction and virulence. As a class I viral fusion protein, hemagglutinin is responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane.

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