FITC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) (0.03% Proclin)

Customer Reviews
Cat. No. / Size
Price
Qty
CD9-FM7A1-200tests (50tests X 4)
$240.00
ETA of in-stock products:2 business days

Product Details

  • Source

    Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) is a mouse monoclonal antibody recombinantly expressed from human 293 cells (HEK293), which provides higher batch consistency and long term security of supply.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    FMC63

  • Isotype

    Mouse IgG2a, Kappa

  • Host Species

    Mouse

  • Reactivity

    Human

  • Immunogen

    Human prolymphocytic leukaemia cell line JVM3

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Specificity

    This product is a specific antibody specifically reacts with CD19 protein.

  • Application

    Flow Cytometry (Detection the expression of CD19 on Human cells).

  • Recommended Dilution

    1:50

  • Form

    Liquid

  • Formulation

    Supplied as 0.2 μm-filtered solution in PBS (pH 7.4) containing 0.03% ProClin 300, with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Shipping

    Liquid product is shipped at blue ice.

  • Storage

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 12 months under sterile conditions.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

Customers Also Viewed

Performance Data

  • Bioactivity-FACS

     CD19 FACS

    Flow cytometric analysis of Human peripheral blood lymphocytes staining with FITC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) (Cat. No. CD9-FM7A1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL), compared with isotype control antibody. FITC signal was used to evaluate the binding activity (QC tested).

    Protocol
  •  CD19 FACS

    Flow cytometric analysis of Human peripheral blood lymphocytes staining with PE anti-human CD3 antibody and FITC-Labeled Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a (FMC63) (Cat. No. CD9-FM7A1) at 1:50 dilution (2 μL of the antibody stock solution corresponds to labeling of 1e6 cells in a final volume of 100 µL). FITC signal was used to evaluate the binding activity (QC tested).

    Protocol

Customer Reviews
Writing Reviews

Background

B-lymphocyte antigen CD19 is also known as CD19 (Cluster of Differentiation 19), is a single-pass type I membrane protein which contains two Ig-like C2-type (immunoglobulin-like) domains. CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase. As on T cells, several surface molecules form the antigen receptor and form a complex on B lymphocytes. The (almost) B cell-specific CD19 phosphoglycoprotein is one of these molecules. The others are CD21 and CD81. These surface immunoglobulin (sIg)-associated molecules facilitate signal transduction. On living B cells, anti-immunoglobulin antibody mimicking exogenous antigen causes CD19 to bind to sIg and internalize with it. The reverse process has not been demonstrated, suggesting that formation of this receptor complex is antigen-induced. This molecular association has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndromes characterized by diminished antibody production. CD19 has been shown to interact with: CD81, CD82, Complement receptor 2, and VAV2.

Recent Advances

 
Drug Development Progress
  • English Name:

    B-lymphocyte antigen CD19

  • Category:

  • Approved Drugs:

    14 Details

  • Drugs in Clinical Trials:

    435 Details

  • Highest Development Stage:

    Approved

Drug Candidate Licensing
More
  • Basic Product Information
  • Product Details
  • Customers Also Viewed
  • Performance Data
  • Customer Reviews
  • Background