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Biotinylated Anti-His Tag Antibody (AY63), Fab, Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
HIS-BVM920-100ug
$263.00
HIS-BVM920-1mg (500ug X 2)
$1764.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Source

    Biotinylated Anti-His Tag Antibody (AY63), Fab, Avitag is a recombinant mouse monoclonal antibody expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    AY63

  • Host Species

    Mouse

  • Reactivity

    His tag (N‑terminal, C‑terminal, internal His‑tagged fusion proteins)

  • Immunogen

    A protein peptide with his tag

  • Conjugate

    Biotin

  • Specificity

    This product is a specific antibody specifically reacts with His tag protein, and can recognizes C-terminal, N-terminal, and internal His tagged fusion proteins.

  • Application

    Application
    Recommended Usage
    ELISA
    1:10-1:100
  • Purification

    Protein A purified / Protein G purified.

  • Aggregation

    Less than 10%, as determined by SEC-MALS.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     His ELISA

    Immobilized Human SOST, His Tag (Cat. No. HST-H5245) at 1 μg/mL (100 μL/well) can bind Biotinylated Anti-His Tag Antibody (AY63), Fab, Avitag (Cat. No. HIS-BVM920) with a linear range of 0.02-5 μg/mL (QC tested).

    Protocol

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Background

His tag is a very small molecular weight tag, usually composed of 6-10 histidine (His). It is one of the commonly used tags for protein purification and detection. Due to its small molecular weight, fusion into the target protein has almost no effect on the structure and characteristics of the protein. Anti-his tag antibody can accurately detect, locate and purify His tag fusion protein, so as to provide convenience for the vast number of researchers.

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