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Human Vinculin / VCL Protein, His Tag

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VIN-H52H9-100ug
$360.00
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VIN-H52H9-1mg
$2,380.00
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VIN-H52H9
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Product Details
Performance Data
Reviews & Citations
Resources
  • Product Details
  • Performance Data
  • Reviews & Citations
  • Important Statement
  • Resources
Synonyms

Vinculin, VCL, MV, Metavinculin

Source

Human Vinculin, His Tag (VIN-H52H9) is expressed from human 293 cells (HEK293). It contains AA Pro 2 - Gln 1066 (Accession # P18206-2).

Predicted N-terminus: Pro 2

Molecular Characterization
Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 118.5 kDa. The protein migrates as 130-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

Vinculin is a ubiquitously expressed actin-binding protein and involves in cross-linking of the cell cytoskeleton with the extracellular matrix for mechanical support of the cell. Vinculin is frequently used as a marker for both cell-cell and cell-extracellular matrix (focal adhesion) adherens-type junctions.