Human Ubiquitin Protein, HA Tag (MALS verified)

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UBN-H5143-100ug
$350.00
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UBN-H5143-500ug
$1,320.00
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UBN-H5143
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Synonyms

HEL-S-50

Source

Human Ubiquitin Protein, HA Tag (UBN-H5143) is expressed from E. coli cells. It contains AA Gln 2 - Gly 76 (Accession # P0CG47-1).

Predicted N-terminus: Met

Molecular Characterization

This protein carries HA tag at the N-terminus.

The protein has a calculated MW of 9.6 kDa. The protein migrates as 8 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris-HCl, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein.