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Product Details
Synonyms
TL1A, VEGI, TNFSF15
Source
Biotinylated Human TL1A Protein, His,Avitag (TLA-H52Q1) is expressed from human 293 cells (HEK293). It contains AA Leu 72- Leu 251 (Accession # O95150-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 24.1 kDa. The protein migrates as 28-32 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human TL1A Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human DcR3, Fc Tag (Cat. No. TNB-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human DcR3, Fc Tag (Cat. No. TNB-H5255) with a linear range of 0.1-3 ng/mL (Routinely tested).
Protocol
Immobilized Human DR3, Fc Tag (Cat. No. DR3-H5253) at 5 μg/mL (100 μL/well) can bind pre-mixed increasing concentrations of Anti-TL1A Neutralizing Antibody, Human lgG1 and 1 μg/mL (50μL/well) Biotinylated Human TL1A, His Avitag (Cat.No.TLA-H52Q1) with a half maximal inhibitory concentration (lC50) of 1.95 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human DR3 Protein, His Tag (Cat. No. TN5-H52H3) immobilized on CM5 Chip can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with an affinity constant of 97.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Anti-TL1A antibody captured on Protein A Chip can bind Biotinylated Human TL1A Protein, His,Avitag (Cat. No. TLA-H52Q1) with an affinity constant of 0.730 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor superfamily member 15
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
18 Details
- Highest Development Stage：
Phase 3 Clinical
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