Synonyms

CD33, SIGLEC3, gp67

Source

Human Siglec-3 Protein, Llama IgG2b Fc Tag (CD3-H5259) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).

Predicted N-terminus: Asp 18

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a llama IgG2b Fc tag at the C-terminus.

The protein has a calculated MW of 54.6 kDa. The protein migrates as 64-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Application

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.

Endotoxin

Less than 0.01 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process