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Product Details
Synonyms
CD33, SIGLEC3, gp67
Source
Human Siglec-3, His Tag (CD3-H5226) is expressed from human 293 cells (HEK293). It contains AA Asp 18 - His 259 (Accession # AAH28152.1).
Predicted N-terminus: Asp 18
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.6 kDa. The protein migrates as 43-55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Siglec-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Siglec-3, His Tag (Cat. No. CD3-H5226) is more than 95% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Anti-Human CD33 MAb, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Human Siglec-3, His Tag (Cat. No. CD3-H5226) at 1 μg/mL (100 μL/well) can bind Lintuzumab with a linear range of 0.06-1 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Anti-Human CD33 MAb, Mouse IgG1 on AMC Biosensor, can bind Human Siglec-3, His Tag (Cat. No. CD3-H5226) with an affinity constant of 1.86 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Myeloid cell surface antigen CD33
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
44 Details
- Highest Development Stage：
Approved
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