RNase H (5U/μl)

RNase H is an endoribonuclease that can specifically hydrolyze RNA in DNA-RNA hybrid chains. RNase H cannot digest single-stranded or double-stranded DNA or RNA.

Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.

One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 pmol of a fluorescently labeled 25 base pair RNA:DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 37℃.

>90% as determined by SDS-PAGE.

Supplied as 0.2 μm filtered solution in 10 mM Tris, 0.1 mM EDTA, 1 mM DTT, 0.2% BSA, pH7.4 with glycerol as protectant.

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This product is supplied and shipped on dry ice.

• The product MUST be stored at -20°C or lower upon receipt.
• -20°C for 6 months under sterile conditions.