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Product Details
Synonyms
Prolactin R, PRLR, PRL-R
Source
Human Prolactin R Protein, His Tag&Tag Free (PRR-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 25 - Asp 234 (Accession # P16471-1).
Predicted N-terminus: Gln 25
Request for sequenceMolecular Characterization
The protein has a calculated MW of 31.1 kDa. The protein migrates as 33-36 kDa and 38-41 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human Prolactin R Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Human Prolactin R Protein, His Tag&Tag Free (Cat. No. PRR-H52H3) is more than 90% and the molecular weight of this protein is around 60-85 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Prolactin Protein, Mouse IgG2a Fc Tag (Cat. No. PRN-H5257) at 5 μg/mL (100 μL/well) can bind Human Prolactin R Protein, His Tag&Tag Free (Cat. No. PRR-H52H3) with a linear range of 0.005-0.156 μg/mL (QC tested).
Protocol
Bioactivity-SPR
Human Prolactin R Protein, His Tag&Tag Free (Cat. No. PRR-H52H3) immobilized on CM5 Chip can bind Human Prolactin Protein, His Tag (Cat. No. PRN-H52H4) with an affinity constant of 1.51 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Prolactin receptor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 3 Clinical
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