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Product Details
Product Details
PNGase F is the most effective enzymatic method for removing almost all N-linked oligosaccharides from glycoproteins. PNGase F is an amidase, which cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides.
Application
Leaves N-glycan core oligosaccharides intact and suitable for further analysis.
Non-recombinant with no detectable endoglycosidase F1, F2 or F3 contamination.Unit Definition
One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl.
Purity
>90% as determined by SDS-PAGE.
Enzyme Activity
>500 U/μL
Notes
To deglycosylate a native glycoprotein, longer incubation time as well as more enzyme may be required.
PNGase F will not cleave N-linked glycans containing core α1-3 Fucose.
Formulation
Supplied as 0.2 μm filtered solution in 20 Mm Tris, 50 mM NaCl, pH7.5 with glycerol as protectant.
Contact us for customized product form or formulation.
Shipping
This product is supplied and shipped with dry ice, please inquire the shipping cost.
Storage
This product is stable after storage at:
- The product MUST be stored at -20°C or lower upon receipt.
- -20°C for 12 months under sterile conditions.
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Performance Data
SDS-PAGE
The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity
One unit enzyme to remove > 95% of the carbohydrate from 10 µg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 20 µl (QC tested).
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