Save Product
Product Details
Synonyms
CD113, Nectin-3, PVRL3
Source
Human Nectin-3, His Tag (PV3-H52E4) is expressed from human 293 cells (HEK293). It contains AA Gly 58 - Asp 400 (Accession # Q9NQS3).
Predicted N-terminus: Gly 58
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 39.6 kDa. The protein migrates as 55-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Human Nectin-3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Human Nectin-3, His Tag (Cat. No. PV3-H52E4) is more than 85% and the molecular weight of this protein is around 65-90 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Nectin-3, His Tag (Cat. No. PV3-H52E4) at 2 μg/mL (100 μL/well) can bind Human PVRL1, Fc Tag with a linear range of 1-20 ng/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) on Protein A Biosensor, can bind Human Nectin-3, His Tag (Cat. No. PV3-H52E4) with an affinity constant of 2.7 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human Nectin-3, His Tag (Cat. No. PV3-H52E4) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 4.99 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Contact Us
Drug Candidate Licensing
