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Product Details
Synonyms
LGR5, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67, G-protein coupled receptor HG38, Leucine-rich repeat-containing G-protein coupled receptor 5
Source
Biotinylated Human LGR5 (22-561) Protein, His,Avitag (LG5-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gly 22 - Arg 561 (Accession # O75473-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 64.1 kDa. The protein migrates as 65-80 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human LGR5 (22-561) Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5 (22-561) Protein, His,Avitag (Cat. No. LG5-H82E4) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Human R-Spondin 3 Protein, Fc Tag (Cat. No. RS3-H5253) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LGR5 (22-561) Protein, His,Avitag (Cat. No. LG5-H82E4) with a linear range of 0.2-5 ng/mL (Routinely tested).
Protocol
Immobilized Petosemtamab at 1 μg/mL (100 μL/well) can bind Biotinylated Human LGR5 (22-561) Protein, His,Avitag (Cat. No. LG5-H82E4) with a linear range of 0.1-8 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Leucine-rich repeat-containing G-protein coupled receptor 5
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 2 Clinical
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