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Product Details
Synonyms
Integrin alpha 5 beta 1, ITGA5&ITGB1
Source
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (IT1-H82Wa) is expressed from human 293 cells (HEK293). It contains AA Phe 42 - Tyr 995 (ITGA5) & Gln 21 - Asp 728 (ITGB1) (Accession # P08648-1 (ITGA5) & P05556-1 (ITGB1)).
Predicted N-terminus: Phe 42 (ITGA5) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of ITGA5 and ITGB1, has a calculated MW of 112.4 kDa (ITGA5) and 83.7 kDa (ITGB1). Subunit ITGA5 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™) and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 100-115 kDa (ITGA5) and 135-150 kDa (ITGB1) respectively due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Recombinant Human Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) with a linear range of 1-16 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Biotinylated Human ITGA5&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82Wa) captured on Biotin CAP-Series S Sensor Chip can bind Human Fibronectin with an affinity constant of 4.4 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha-5/beta-1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Approved
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