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IL-23 alpha & IL-12 beta
Cynomolgus IL23A & IL12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 20.3 kDa (IL23A) and 34.8 kDa (IL12B). Subunit IL-23A is fused with a polyhistidine tag at the C-terminus and subunit IL-12B contains no tag. The protein migrates as 22-25 kDa,38-39 kDa and 41-43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Cynomolgus IL23A & IL12B Heterodimer Protein, His Tag&Tag Free(ILB-C52W3) was digested with trypsin & chymotrypsin and characterized by LC-MS/MS peptide mapping, achieving 100% sequence coverage (Routinely tested).
Less than 1.0 EU per μg by the LAL method / rFC method.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
