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Product Details
Synonyms
IL-23 R
Source
Biotinylated Human IL-23 R, Fc,Avitag (ILR-H82F3) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Gly 355 (Accession # Q5VWK5-1).
Predicted N-terminus: Gly 24
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-105 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human IL-23 R, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) is more than 85% and the molecular weight of this protein is around 165-195 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-H52W5) with a linear range of 39-156 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) at 5 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Human IL23A/p19 Neutralizing Antibody and 0.25 μg/mL (100 μL/well) Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-H52W5) with a half maximal inhibitory concentration (IC50) of 0.3889 μg/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human IL-23 R, Fc,Avitag (Cat. No. ILR-H82F3) captured on Biotin CAP-Series S Sensor Chip can bind Rat IL-23 alpha& IL-12 beta Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-R52W3) with an affinity constant of 0.503 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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