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Product Details
Synonyms
IL-17F, Interleukin-17F, Cytokine ML-1
Source
Cynomolgus IL-17F, His Tag (ILF-C5243) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # G7P4V0-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag with a linear range of 0.005-0.313 μg/mL (QC tested).
Protocol
Immobilized Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) at 2 μg/mL (100 μL/well) can bind Recombinant Human IL-17RC Isoform 1 Fc Chimera with a linear range of 0.5-16 ng/mL (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Human IL-17 RA, Fc Tag on Protein A Biosensor, can bind Cynomolgus IL-17F, His Tag (Cat. No. ILF-C5243) with an affinity constant of 279 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-17F
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
5 Details
- Highest Development Stage：
NDA/BLA
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