Biotinylated Mouse Her2 / ErbB2 Protein, Fc,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
HE2-M82F3-25ug
$370.00
HE2-M82F3-200ug
$1465.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    ERBB2, CD340, HER-2, neu, HER2, MLN19, NEU, NGL, TKR1

  • Source

    Biotinylated Mouse Her2 Protein, Fc,Avitag (HE2-M82F3) is expressed from human 293 cells (HEK293). It contains AA Thr 23 - Thr 653 (Accession # P70424).

    Predicted N-terminus: Thr 23

    Request for sequence
  • Molecular Characterization

    Her2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 97.8 kDa. The protein migrates as 110-130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Her2 SDS-PAGE

    Biotinylated Mouse Her2 Protein, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    Her2 SEC-MALS

    The purity of Biotinylated Mouse Her2 Protein, Fc,Avitag (Cat. No. HE2-M82F3) is more than 95% and the molecular weight of this protein is around 210-240 kDa verified by SEC-MALS.

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Background

Human Epidermal growth factor Receptor 2 (HER2) is also called ERBB2, HER-2,HER-2 /neu, NEU, NGL,TKR1 and c-erb B2,and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. Approximately 30% of breast cancers have an amplification of the HER2 gene or overexpression of its protein product. Overexpression of this receptor in breast cancer is associated with increased disease recurrence and worse prognosis. HER2 appears to play roles in development, cancer, communication at the neuromuscular junction and regulation of cell growth and differentiation .

Recent Advances

 
Drug Development Progress
  • English Name:

    Receptor protein-tyrosine kinase erbB-2

  • Category:

  • Approved Drugs:

    47 Details

  • Drugs in Clinical Trials:

    238 Details

  • Highest Development Stage:

    Approved

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