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Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 / EBV GP350 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
GP0-E52H6-100ug
$430.00
GP0-E52H6-1mg
$2360.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    Epstein-Barr virus (Herpesvirus 4), EBV Glycoprotein gp350, EBV GP350

  • Source

    Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag (GP0-E52H6) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Thr 470 (Accession # P03200-1).

    Predicted N-terminus: Met 1

    Request for sequence
  • Molecular Characterization

    gp350 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 52.3 kDa. The protein migrates as 80-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with Trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    gp350 SDS-PAGE

    Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    gp350 SEC-MALS

    The purity of Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag (Cat. No. GP0-E52H6) is more than 95% and the molecular weight of this protein is around 85-95 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     gp350 ELISA

    Immobilized Epstein-Barr virus (Herpesvirus 4) EBV Glycoprotein gp350 Protein, His Tag (Cat. No. GP0-E52H6) at 1 μg/mL (100 μL/well) can bind various dilution ratio of EBV-gp350 Antibody (Routinely tested).

    Protocol

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Background

Epstein-Barr virus (EBV), also designated human herpesvirus 4 (HHV-4), is a member of the herpesvirus family and is one of the most common human viruses. EBV binds to the cell surface receptor 2 (CR2) on human B cells using its major envelope glycoprotein 350 (gp350) and, as such, the EBV gp350 Envelope Protein, also designated the EBV envelope glycoprotein complex 250/350, is crucial in mediating the initial stages of EBV infection. The EBV gp350 Envelope Protein is expressed on virion envelope as well as EBV producer cells.

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  • Performance Data
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  • Background