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Product Details
Synonyms
GDF-8, Myostatin, GDF8, MSTN, Growth, differentiation factor 8
Source
Biotinylated Human GDF-8 Protein, Avitag (GD8-H8313) is expressed from CHO cells. It contains AA Asp 267 - Ser 375 (Accession # O14793-1).
Predicted N-terminus: Asp 267
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus.
The protein has a calculated MW of 14.4 kDa. The protein migrates as 13-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM HAC, pH3.0 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human GDF-8 Protein, Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Biotinylated Human GDF-8 Protein, Avitag (Cat. No. GD8-H8313) at 0.1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Activin RIIB, His Tag (Cat. No. ACB-H5226) with a linear range of 2-78 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human GDF-8 Protein, Avitag (Cat. No. GD8-H8313) at 0.1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Follistatin Protein, Fc Tag (Cat. No. FON-H5256) with a linear range of 0.3-10 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) immobilized on CM5 Chip can bind Biotinylated Human GDF-8 Protein, Avitag (Cat. No. GD8-H8313) with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Growth/differentiation factor 8
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
NDA/BLA
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