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Product Details
Synonyms
Flt-3, Flk-2, STK-1, CD135, FLK2, FLT-3
Source
Biotinylated Human Flt-3, His,Avitag (FL3-H82E3) is expressed from human 293 cells (HEK293). It contains AA Asn 27 - Ser 543 (Accession # P36888-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 62.1 kDa. The protein migrates as 80-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human Flt-3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human Flt-3, His,Avitag (Cat. No. FL3-H82E3) is more than 90% and the molecular weight of this protein is around 80-110 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human Flt-3 Ligand, His Tag (Cat. No. FLL-H5223) at 1 μg/mL (100 μL/well) can bind Biotinylated Human Flt-3, His,Avitag (Cat. No. FL3-H82E3) with a linear range of 0.039-1.25 μg/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tyrosine-protein kinase receptor FLT3
- Category：
- Approved Drugs：
14 Details
- Drugs in Clinical Trials：
59 Details
- Highest Development Stage：
Approved
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