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Product Details
Synonyms
FGF1, AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-alpha, FGFA, GLIO703, HBGF1
Source
Human FGF acidic, Tag Free (AFF-H4116) is expressed from E. coli cells. It contains AA Phe 16 - Asp 155 (Accession # P05230-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 16.0 kDa. The protein migrates as 16 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human FGF acidic, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-SPR
Human FGF R1, His Tag (Cat. No. FG1-H5223) captured on CM5 chip via anti-His antibody can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 55.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) immobilized on CM5 Chip can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 5.91 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF R3 (IIIc), Fc Tag immobilized (Cat. No. FGC-H5256) on CM5 Chip can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 0.44 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human FGF acidic, Tag Free (Cat. No. AFF-H4116) immobilized on CM5 Chip can bind Human Glypican 2, Fc Tag (Cat. No. GP2-H5255) with an affinity constant of 20.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-BLI
Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 186 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Fibroblast growth factor 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 3 Clinical
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