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Product Details
Synonyms
EGF, URG, HOMG4
Source
Alexa Fluor 488-Labeled Human EGF Protein, His Tag (EGF-HA243) is produced via conjugation of AF488 to Human EGF, His Tag with a new generation site-specific technology under Star Staining labeling platform. Human EGF, His Tag is expressed from human 293 cells (HEK293). It contains AA Asn 971 - Arg 1023 (Accession # P01133-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human EGF Protein, His Tag (Cat. No. EGF-HA243) at 5 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag (Cat. No. EGR-H5252) with a linear range of 0.05-1 ng/mL (Routinely tested).
Protocol
Bioactivity-FACS
2e5 of HEK293/Human EGF R Stable Cell Line (Cat. No. CHEK-ATP148) were stained with 100 μL of 0.3 μg/mL of Alexa Fluor 488-Labeled Human EGF Protein, His Tag (Cat. No. EGF-HA243) and negative control protein respectively, washed and then analyzed with FACS (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Epidermal growth factor
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Phase 2 Clinical
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