Human DLL1 / Delta1 Protein, Fc Tag, premium grade

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Cat. No. / Size
Price
Qty
DL1-H5259-100ug
$260.00
DL1-H5259-1mg (500ug X 2)
$1815.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    DLL1, Delta1, H-Delta-1

  • Source

    Human DLL1 Protein, Fc Tag, premium grade (DL1-H5259) is expressed from human 293 cells (HEK293). It contains AA Gln 18 - Gly 540 (Accession # O00548-1).

    Predicted N-terminus: Gln 18

    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.

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  • Molecular Characterization

    DLL1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 82.8 kDa. The protein migrates as 103 kDa±5 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 0.01 EU per μg by the LAL method / rFC method.

  • Sterility

    Negative

  • Mycoplasma

    Negative

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    DLL1 SDS-PAGE

    Human DLL1 Protein, Fc Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    DLL1 SEC-MALS

    The purity of Human DLL1 Protein, Fc Tag, premium grade (Cat. No. DL1-H5259) is more than 90% and the molecular weight of this protein is around 165-205 kDa verified by SEC-MALS.

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  • Bioactivity-ELISA

     DLL1 ELISA

    Immobilized Human DLL1 Protein, Fc Tag, premium grade (Cat. No. DL1-H5259) at 10 μg/mL (100 μL/well) can bind Biotinylated Human NOTCH1 Protein, Fc Tag with a linear range of 0.02-2.5 μg/mL (QC tested).

    Protocol

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Background

Delta-like protein 1 (DLL1) is also known as Drosophila Delta homolog 1 (Delta1 or H-Delta-1), which contains one DSL domain and eight EGF-like domains. DLL1 is ubiquitinated by MIB (MIB1 or MIB2), leading to its endocytosis and subsequent degradation. As for expression, DLL1 is expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney. Furthermore, DLL1 acts as a ligand for Notch receptors. Also, DLL1 can block the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Recent Advances

 
Drug Development Progress
  • English Name:

    Delta Like Canonical Notch Ligand 1

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

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  • Highest Development Stage:

    Preclinical

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  • Background