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Human CTGF / CCN2 Protein, His Tag

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Cat. No. / Size
Price
Qty
CTF-H52H5-100ug
$395.00
CTF-H52H5-1mg (250ug X 4)
$2615.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    CCN2, NOV2, HCS24, IGFBP8, IBP-8, IGFBP-8, IGF-binding protein 8

  • Source

    Human CTGF, His Tag (CTF-H52H5) is expressed from human 293 cells (HEK293). It contains AA Gln 27 - Ala 349 (Accession # Q5M8T4-1).

    Predicted N-terminus: Gln 27

    Request for sequence
  • Molecular Characterization

    CTGF Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 37.4 kDa. The protein migrates as 36-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Potassium glutamate and Arginine, pH7.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CTGF SDS-PAGE

    Human CTGF, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     CTGF ELISA

    Immobilized Human CTGF, His Tag (Cat. No. CTF-H52H5) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CTGF Antibody, Human IgG1 with a linear range of 0.3-10 ng/mL (QC tested).

    Protocol
  • Bioactivity-BLI

     CTGF BLI

    Loaded Monoclonal Anti-Human CTGF Antibody, Human IgG1 on AHC Biosensor, can bind Human CTGF, His Tag (Cat. No. CTF-H52H5) with an affinity constant of 6.29 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

    Protocol

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Background

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a member of the CCN (CCN1-6) family of modular matricellular proteins. Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain. CTGF promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced D synthesis. Analysis of CCN2 function in vivo has focused primarily on its key role as a mediator of excess ECM synthesis in multiple fibrotic diseases.

Recent Advances

 
Drug Development Progress
  • English Name:

    Connective tissue growth factor

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    5 Details

  • Highest Development Stage:

    Approved

Drug Candidate Licensing
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  • Background