Biotinylated Human CA125 / MUC16 (13810-14451) Protein, Fc,Avitag™

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Cat. No. / Size
Price
Qty
CA5-H82F8-25ug
$415.00
CA5-H82F8-200ug
$1880.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, mucin-16

  • Source

    Biotinylated Human CA125 (13810-14451), Fc,Avitag (CA5-H82F8) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).

    Predicted N-terminus: Pro 13810

    Request for sequence
  • Molecular Characterization

    CA125 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 100.0 kDa. The protein migrates as 120-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Endotoxin

    Less than 0.1 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CA125 SDS-PAGE

    Biotinylated Human CA125 (13810-14451), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • Bioactivity-ELISA

     CA125 ELISA

    Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CA125 (13810-14451), Fc,Avitag (Cat. No. CA5-H82F8) with a linear range of 0.6-78 ng/mL (QC tested).

    Protocol

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Background

The CA125, also known as the MUC16, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy. The CA 125 molecule is almost certainly a glycoprotein with a predominance of O-linkages. It is heterogeneous with regard to both size and charge, most likely due to continuous deglycosylation of side chains during its life-span in bodily fluids. It exists as a very large complex (perhaps as much as 4 million daltons) under natural conditions.

Recent Advances

 
Drug Development Progress
  • English Name:

    Mucin-16

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    7 Details

  • Highest Development Stage:

    Phase 3 Clinical

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