Product Details
Synonyms
CA125, CA-125, CA125MUC-16, FLJ14303, MUC16, mucin 16, mucin-16
Source
Biotinylated Human CA125 (13810-14451), Fc,Avitag (CA5-H82F8) is expressed from human 293 cells (HEK293). It contains AA Pro 13810 - Pro 14451 (Accession # Q8WXI7-1).
Predicted N-terminus: Pro 13810
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 100.0 kDa. The protein migrates as 120-160 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Biotinylated Human CA125 (13810-14451), Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA

Immobilized Human Mesothelin (296-580), Fc Tag (Cat. No. MSN-H526x) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CA125 (13810-14451), Fc,Avitag (Cat. No. CA5-H82F8) with a linear range of 0.6-78 ng/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Mucin-16
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
7 Details
- Highest Development Stage:
Phase 3 Clinical



















