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Biotinylated Human BTN3A2 Protein, His,Avitag™

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Cat. No. / Size
Price
Qty
BT2-H82E7-25ug
$360.00
BT2-H82E7-200ug
$1420.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    BT3.2, BT3.3, BTF3, BTF4

  • Source

    Biotinylated Human BTN3A2, His,Avitag (BT2-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Trp 248 (Accession # P78410-1).

    Predicted N-terminus: Gln30

    Request for sequence
  • Molecular Characterization

    BTN3A2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 27.3 kDa. The protein migrates as 30-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    BTN3A2 SDS-PAGE

    Biotinylated Human BTN3A2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Customer Reviews
Writing Reviews

  • S***y2022-12-05
    Likes
    Purchased this reagent and the initial QC showed very good purity. Past the initial QC check, Used this reagent for ELISA and the results are specific compared to other reagents. Also, it has been a reliable protein for screening purposes. Initial screening with this reagent has shown high specificity. Very much satisfied with the results and I highly recommend this reagent for screening and ELISA and even in the immunization experiments.

Background

Butyrophilin subfamily 3 member A2(BTN3A2/BT3.2) mR expression by tumoral cells was previously identified as a prognostic factor in a small cohort of high grade serous epithelial ovarian cancer (HG-EOC). We find that the three BTN3A isoforms: BTN3A1, BTN3A2, and BTN3A3, have high structural homology to the B7 superfamily of proteins and exist as V-shaped homodimers in solution, associating through the membrane proximal C-type Ig domain.

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