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Product Details
Synonyms
B7-H4, VTCN1, B7S1, B7h.5
Source
Biotinylated Human B7-H4 Protein, Avitag,His Tag (B74-H82E2) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Ala 258 (Accession # NP_078902).
Predicted N-terminus: Phe 29
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 28 kDa. The protein migrates as 43-57 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Application
B74-H82E2 works best for experiments that test the binding between B7-H4 and candidate antibodies, such as biopanning and other relevant assays.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated Human B7-H4 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human B7-H4 Protein, Avitag,His Tag (Cat. No. B74-H82E2) is more than 85% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Anti-Human B7-H4 MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human B7-H4 Protein, Avitag,His Tag (Cat. No. B74-H82E2) with a linear range of 3-48 ng/mL (QC tested).
Protocol
Customer Reviews Writing Reviews
1***12019-11-16
LikesWe purchased total of 600ug of this biotin-labeled antigen for liquid phase panning and antibody screening of affinity maturation. This antigen has good stability, high quality, and high repeatability for each experiment. Using this antigen, we screened high-affinity antibodies against this target. And using this antigen for screening of affinity maturation, we obtained antibodies with 10-times increased affinity. I highly recommended this product.
Preguntas Frecuentes
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
V-set domain-containing T-cell activation inhibitor 1
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
11 Details
- Highest Development Stage：
Phase 3 Clinical
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