Anti-Doxorubicin Antibody Screening Panel

Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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APA-05-40ug
$260.00
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Product Details

This antibody panel comprises two distinct rabbit monoclonal antibodies against Doxorubicin, with slight differences in their recognition epitopes. Consequently, they may exhibit varying affinity levels towards different Doxorubicin analogs or different conjugates of Doxorubicin and linkers. This panel can be used to conduct preliminary experiments for screening the optimal antibody clone.

Components
Cat.No.
Clone
Size
DON-MY2216
1M2C3
20 ug
DON-MY2215
1M2B1
20 ug
Specificity

Specifically recognizes Doxorubicin.

Source

This panel contains Two anti-Doxorubicin monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with Doxorubicin as the payload. All the anti-Doxorubicin monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.

Isotype

Rabbit IgG, Kappa

Purification

Protein A purified.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

Doxorubicin a cytotoxic anthracycline antibiotic, is an anti-cancer chemotherapy agent. As the payload of ADC drugs, Doxorubicin can act directly on DNA, insert into the double helix strands of DNA, cause the latter to unwind, change the template properties of DNA, and inhibit DNA polymerase, thereby inhibiting the synthesis of both DNA and RNA. Anti-Doxorubicin antibody is a rabbit monoclonal antibody specifically reacts with Doxorubin without other payloads, which is more sensitive than mouse antibody. The anti-Doxorubicin antibody is a useful reagent in PK assay to determine conjugated antibodies.