Product Details
FAB
- Comprehensive validation - Rigorously validated through comprehensive methodology to ensure reliable and accurate results.
- Simple and fast operation - No complicated washing steps, significantly reducing time.
- High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
- Accurate and reliable results - High sensitivity with minimal matrix effects.
- High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
- Fast completion - Results in just 2 hours.
Product Overview
Mouse TNF-alpha Detection Kit (TR-FRET) enables sensitive and rapid detection of mouse TNF-alpha in a homogeneous system within 2 hours. Designed for ease of use and high-throughput screening, it is fully compatible with microplate formats, allowing efficient analysis of large sample volumes. This kit is specifically designed for the accurate quantitation of mouse TNF-alpha in cell culture supernatant, serum, and plasma.
Product Specifications
Assay TypeSandwich-TR-FRETAnalyteMouse TNF-alphaReactivityMouseRegulatory StatusRUOSensitivityLOQ=39.063 pg/mLStandard Curve Range39.063 pg/mL-5000 pg/mLAssay Time2 hrSuitable Sample TypeFor Mouse TNF-alpha detection in human serum, human plasma,cell culture supernatantsSample volume16 μLElevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
Workflow

Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
Materials Provided
IDComponentsSizeFRTB102-C01Anti-Mouse TNF-alpha Antibody Europium-chelate100 tests/500 testsFRTB102-C02FA Labeled Anti-Mouse TNF-alpha Antibody100 tests/500 testsFRTB102-C03Mouse TNF-alpha Standard5 μg/100 tests
5 μg/500 testsDB-04TR-FRET Sample Dilution Buffer, pH7.450 mL/100 tests & 500 testsDB-05TR-FRET Detection Buffer, pH7.450 mL/100 tests & 500 testsACRO Quality Management System
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Performance Data
Dilution Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse TNF-alpha standard were serially diluted with TR-FRET Sample Dilution Buffer, pH 7.4 (DB-04), to generate samples with concentrations within the dynamic range of the assay.

Intra-Assay Statistics
Five samples with known concentrations were tested repeatedly 16 times within a single analytical batch to assess within-batch precision and accuracy, Intra-Assay Precision CV≤30%, Intra-Assay Accuracy Recovery: 70%-130%.

Inter-Assay Statistics
Five samples with known concentrations were tested repeatedly across three independent analytical batches to evaluate between-batch precision and accuracy. Inter-Assay Precision CV≤30%, Inter-Assay Accuracy Recovery: 70%-130%.

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Recent Advances
- English Name:
Tumor necrosis factor α
- Category:
- Approved Drugs:
68 Details
- Drugs in Clinical Trials:
74 Details
- Highest Development Stage:
Approved



