Product Details
Materials Provided
IDComponentsSizeBIS006-C01Pre-coated Human EGFR Protein Microplate1 plate (8×12 strips)BIS006-C02Anti-EGFR & c-Met Antibody Standard20 μgBIS006-C03Biotin-Human c-Met Protein30 μgBIS006-C04Streptavidin-HRP20 μgBIS006-C0510×Washing Buffer50 mLBIS006-C062×Dilution Buffer50 mLBIS006-C07Substrate Solution12 mLBIS006-C08Stop Solution7 mLProduct Overview
The Bispecific EGFR:c-Met Bridging ELISA Kit is a comprehensive, ready-to-use assay designed to functionally confirm the simultaneous binding of anti-EGFR-anti-c-Met bispecific antibodies (BsAbs) to both of their intended targets. This assay quantifies the formation of the critical EGFR-BsAb-c-Met trimolecular complex, providing a direct measure of the antibody's primary mechanism of action. This all-inclusive kit contains all essential reagents for 100 reactions, including recombinant human EGFR, a c-Met-containing detection reagent, and a validated positive control antibody, thereby streamlining the screening and profiling of therapeutic candidates with exceptional precision and reliability.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.
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Performance Data
Typical Data
Please refer to DS document for the assay protocol.

Detection of anti-EGFR & c-Met antibodies by Bridging ELISA Assay.
Bispecific Human EGFR & c-Met Bridging ELISA Kit can specifically identify Anti-EGFR & c-Met Antibodies, with linear range of 0.013-0.431 nM (QC tested).
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