Human SCF / CD117 Inhibitor Screening Kit (TR-FRET)

Product Details

FAB
1. Comprehensive validation - Validated with various antibody drugs.
2. Simple and fast operation - No complicated washing steps, significantly reducing time.
3. High batch consistency - Strict control over raw materials and finished product quality, ensuring a stable supply.
4. Accurate and reliable results - High sensitivity with minimal matrix effects.
5. High throughput capability - Support multiple product specifications, ideal for high-throughput screening.
6. Fast completion - Results in just 1 hour.
Product Specifications
Assay Type
Competition-TR-FRET
Reactivity
Human
Regulatory Status
RUO
Assay Time
1 h
Sample volume
10 μL
Product Overview
The Human SCF / CD117 Inhibitor Screening Kit (TR-FRET) is based on a homogeneous (no wash) competition TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer) to screen for inhibitors of human SCF binding to CD117 within one hour. It can also be used as a universal detection tool to identify the ability of human SCF to bind to human CD117.
Storage
1. Store the unopened kit at 2-8°C upon receipt.
2. Locate the expiration date on the outer packaging and do not use reagents beyond their expiration date.
3. The opened kit should be stored according to the storage conditions listed in the Components Table. The shelf life of the opened kit is 30 days from the date of opening.

ID	Components	Size
FRTP044-C01	Human SCF Protein Europium-chelate	100 tests/500 tests
FRTP044-C02	FA-Labeled Human CD117 Protein	100 tests/500 tests
FRTP044-C03	Anti-CD117 Neutralizing Antibody	30 μg/100 tests 150 μg/500 tests
DB-04	TR-FRET Sample Dilution Buffer, pH7.4	50 mL/100 tests & 500 tests
DB-05	TR-FRET Detection Buffer, pH7.4	50 mL/100 tests & 500 tests

Assay Principles
This Human SCF / CD117 Inhibitor Screening Kit (TR-FRET) is based on TR-FRET technology (Time-Resolved Fluorescence Resonance Energy Transfer). This assay uses the mixture of biotinylated human SCF and Europium-chelate labeled streptavidin as the Donor and FA-Labeled Human CD117 Protein.
— When the sample does not contain the inhibitors that block the binding of human SCF to human CD117, the Donor and Acceptor are in close proximity because of the binding of human SCF and human CD117. Upon Donor excitation with light of a specific wavelength (337 nm), in addition to Donor emission (620 nm), non-radiative transfer of energy occurs between Donor and Acceptor, resulting in Acceptor emission (665 nm).
— When the sample contains the inhibitors that block the binding of human SCF to human CD117, the components inhibit the binding between the Donor and Acceptor and thereby prevent FRET from occurring.
ACRO Quality Management System

Bioactivity-TR-FRET
Please refer to DS document for the assay protocol.
In this TR-FRET assay, Human SCF Protein Europium-chelate is used as the Donor and FA-Labeled Human CD117 Protein is used as the Acceptor. An inhibition Assay was performed to evaluate the interaction between human SCF and human CD117 using Anti-CD117 Neutralizing Antibody resulting in a typical IC50 of 1.381 nM (QC tested).
The kit is suitable for the detection and characterization of SCF / CD117 inhibitors. It was shown that the Anti CD117 antibody (Briquilimab) and Anti-CD117 antibody (Barzolvolimab) disrupted the interaction, with an IC50 of 1.347 & 14.72 nM. The anti-TNF-alpha antibody (Adalimumab) showed no significant inhibitory effect in the assay as expected.

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