Human TLR3 (Luc) HEK293 Reporter Cell

Bioactivity Detection of dsRNA with different lengths (RLU).
The Human TLR3 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Lipofectamine™ RNAiMAX-complexed dsRNA samples with different lengths (60, 142, 400 bp) and a dsRNA ladder (21–500 bp). The induction fold exceeded 5 at approximately 32 ng/mL for 60 bp dsRNA, 0.09 ng/mL for 142 bp dsRNA, 0.09 ng/mL for 400 bp dsRNA, and 1.3 ng/mL for a dsRNA ladder (21–500 bp).

Bioactivity Detection of dsRNA with different lengths (FOLD).
The Human TLR3 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Lipofectamine™ RNAiMAX-complexed dsRNA samples with different lengths (60, 142, 400 bp) and a dsRNA ladder (21–500 bp). The induction fold exceeded 5 at approximately 32 ng/mL for 60 bp dsRNA, 0.09 ng/mL for 142 bp dsRNA, 0.09 ng/mL for 400 bp dsRNA, and 1.3 ng/mL for a dsRNA ladder (21–500 bp).

Response to dsRNA (RLU).
The Human TLR3 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Poly(I:C) LMW (0.2-1kb), Poly(I:C) HMW (1.5-8kb), ssRNA and DNA complexed with Lipofectamine™ RNAiMAX. The reporter cell showed strong and dose-dependent activation in response to both Poly(I:C) LMW (0.2-1kb) and Poly(I:C) HMW (1.5-8kb), with EC50 of approximately 24.47 ng/mL and 11.31 ng/mL, respectively. In contrast, neither ssRNA nor DNA induced detectable luciferase activation across the tested concentration range (up to 1,000 ng/mL), demonstrating the dsRNA-specific responsiveness of this reporter cell.

Response to dsRNA (FOLD).
The Human TLR3 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Poly(I:C) LMW (0.2-1kb), Poly(I:C) HMW (1.5-8kb), ssRNA and DNA complexed with Lipofectamine™ RNAiMAX. The reporter cell showed strong and dose-dependent activation in response to both Poly(I:C) LMW (0.2-1kb) and Poly(I:C) HMW (1.5-8kb), with max induction folds of approximately 1250.01 and 882.35, respectively. In contrast, neither ssRNA nor DNA induced detectable luciferase activation across the tested concentration range (up to 1,000 ng/mL), demonstrating the dsRNA-specific responsiveness of this reporter cell.

Passage stability analysis by Signaling Bioassay.
The continuously growing Human TLR3 (Luc) HEK293 Reporter Cell was stimulated with serial dilutions of Lipofectamine™ RNAiMAX-complexed 142bp dsRNA. The 142bp dsRNA stimulated response demonstrates passage stabilization (fold induction and EC50) across passage 8-26.