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Product Details
- ProductSizeAmountCellectra™ Human CD3 NanoSort Microbeads, Research grade2 mLFor 10⁹ total cells
Product Description
Cellectra™ Human CD3 NanoSort Microbeads, Research grade are dextran-coated superparamagnetic iron oxide nanoparticles of which the surface is conjugated with recombinant monoclonal antibodies specific to human CD3 (Isotype: mouse IgG1). They are especially designed for the positive selection of CD3+ cells.
Cellectra™ Human CD3 NanoSort Microbeads, Research grade are produced under sterile manufacturing conditions (ISO 5), and no animal- or human-derived components are used throughout the production process. It is produced under our rigorous quality control system that includes a comprehensive set of tests including sterility and endotoxin tests.Application
Cellectra™ Human CD3 NanoSort Microbeads, Research grade are designed for the positive selection of CD3⁺ cells from fresh or frozen human peripheral blood mononuclear cells (PBMCs). CD3E (CD3 epsilon) is an immunoglobulin superfamily protein expressed on mature T lymphocytes and majority of thymocytes. CD3⁺ enrichment enables the isolation of intact total T lymphocytes, which are applicable for ex vivo expansion, CAR-T raw material preparation, T cell functional analysis, downstream subset purification, single cell sequencing, and immunotherapeutic drug screening in tumor immunity and autoimmune disease research. The magnetic Microbeads are conjugated with monoclonal antibodies targeting human CD3 protein, enabling CD3+ cells to be labeled with the specific antibodies and magnetic particles, and separated using the separation columns and magnets.
Storage
For long term storage, the product should be stored in liquid state at -20°C or below Stability upon receipt: 1.Stable for 24 months at -20 °C or below; 2.Stable for 6 months at 2–8 °C under sterile conditions.
Please protect from light and avoid repeated freeze-thaw cycles.
Sterility
Negative
Endotoxin
Less than 2 EU per mL by the LAL method / rFC method.
Mycoplasma
Negative
Important Note
This product is for research use only and not intended for therapeutic or in vivo diagnostic use.
Formulation
Supplied in PBS buffer containing EDTA, rHSA and Poloxamer 188.
ACRO Quality Management System
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Performance Data
Application Data
Superior the positive-selected Cell Separation Performance of CD3+ Cells (Comparison with Company M)
Separation performance was evaluated using samples containing 1×10⁷ human PBMCs, processed with CD3 microbeads from Acro, Company M at recommended dosages. Positive and negative fractions were collected, and 1×10⁶ cells from the flow through fraction were immunostained with CD3 Antibody (FITC-Labeled Monoclonal Anti-Human CD3 Antibody) and 7-AAD, followed by flow cytometric analysis. Data indicated that Acro microbeads achieved a final purity of 99.19% (baseline: 67.48%). These findings validate outstanding separation performance, confirming an optimal balance of purity and recovery relative to Company M benchmarks.
Comparison of the positive-selected Cell Separation Performances
Isolation efficacy was validated using samples containing 1×10⁷ human PBMCs processed with the recommended dosage of Cellectra™ Human CD3 NanoSort Microbeads, Research grade, in parallel with Company M. Comparative analysis demonstrated that Cellectra™ Human CD3 NanoSort Microbeads achieved higher cell recovery rates than Company M, while purity and cell viability were comparable to Company M.
CD3 positive-selected Cell Separation Performances after Freeze-Thawing and Accelerated Aging at 37℃
Freeze-thaw stability was verified by subjecting the Cellectra™ Human CD3 NanoSort Microbeads, Research grade to 3 cycles prior to separation testing. Comparative analysis against the standard batch confirmed that purity and cell recovery were maintained at baseline specifications, demonstrating robust stability. To ensure prolonged shelf life, storage at -20°C or below is recommended upon receipt (however, repeated freeze-thaw cycles are not advised for routine operational use). Accelerated aging studies were conducted at 37°C to evaluate long-term stability profiles. Following 3.5 and 7-day incubation periods, the microbeads sustained high purity and cell recovery performance during PBMC separation. These data substantiate product stability and validate the established shelf-life specifications.
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Recent Advances
Drug Development Progress- English Name：
T cell surface glycoprotein CD3
- Category：
- Approved Drugs：
10 Details
- Drugs in Clinical Trials：
198 Details
- Highest Development Stage：
Approved
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