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Product Details
Source
Chimeric Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 is recombinantly expressed in CHO. It consists of mouse variable regions genetically fused to mouse IgG1 constant domains.
Antibody Type
Recombinant Monoclonal
Clone
AS307
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Conjugate
Biotinylated
Application
ApplicationRecommended Use / PerformanceELISA1:3000-1:30000Purification
Protein A purified.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
ACRO Quality Management System
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Performance Data
Bioactivity-ELISA
Immobilized Human IgG1 Kappa Isotype Control (mAb) (Cat. No. DNP-M2) at 1 μg/mL (100 μL/well) can bind Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-BLS307) with a linear range of 0.03-4 ng/mL (QC tested).
Protocol
Binding activity of Biotinylated Monoclonal Anti-Human-IgG-Fc Antibody, Mouse IgG1 (Cat. No. IGG-BLS307) to different Human IgGs, as determined by ELISA. Human IgGs were coated at 1 μg/mL. The primary antibody (Cat. No. IGG-BLS307) was serially diluted starting from 62.5 ng/mL, and Streptavidin Protein-HRP, Horseradish peroxidase conjugated Streptavidin (Acro, Cat. No. STN-NH913) was used as the secondary antibody at a dilution of 0.1 μg/mL. Binding was measured by OD₄₅₀ (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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