Alexa Fluor™ 647-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45)Star Staining

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FM3-AM534-25tests
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Source

Alexa Fluor 647-Labeled Monoclonal Anti-FMC63 Antibody, Mouse IgG1 (Y45) is a neutralizing antibody produced via conjugation of AF647 to Monoclonal Anti-FMC63 Antibody, Mouse IgG1 under optimal conditions with a new generation site-specific technology under Star Staining labeling platform.

Clone

Y45

Isotype

Mouse IgG1, Kappa

Host Species

Mouse

Reactivity

Mouse

Immunogen

Recombinant FMC63 scFv derived from HEK293 cells

Conjugate

AF647

Excitation Wavelength: 640 nm

Emission Wavelength: 672 nm

Specificity

Specifically recognizes the antigen-recognition domain of FMC63 derived CARs.

Application

Flow Cytometry (Evaluation of Anti-CD19 (FMC63 scFv) CAR Expression).

Recommended Dilution

1:50

Form

Lyophilized

Formulation

Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

Please contact us for customized product forms or formulations.

Reconstitution

Please refer to the Certificate of Analysis (CoA) for specific instructions.

Shipping

Lyophilized product is shipped at ambient temperature.

Storage

For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 24 months in lyophilized state;
  • -70°C for 12 months under sterile conditions after reconstitution.
Notices

Product Specific Notices: For research use only.

Background

FMC63 is an IgG2a mouse monoclonal antibody specific for CD19, which is a target for the immunotherapy of B lineage leukaemias and lymphomas. FMC63 scFv is the most commonly used ectodomain component of CD19-specific CARs. So far, most of reported CART19 trials contain the anti-CD19 scFv derived from FMC63, including the two FDA-approved CARs Kymriah and Yescarta.