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Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1E06)

  • Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells
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Cat. No. / Size
Price
Qty
DUN-MY2287-100ug
$315.00
DUN-MY2287-1mg (500ug X 2)
$1890.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Source

    Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1E06) is a rabbit monoclonal antibody recombinantly expressed in HEK293 cells.

  • Antibody Type

    Recombinant Monoclonal

  • Clone

    M1E06

  • Isotype

    Rabbit IgG, Kappa

  • Immunogen

    Duocarmycin-BSA

  • Specificity

    Specifically recognizes the target-Duocarmycin.

  • Purification

    Protein A purified / Protein G purified.

  • Concentration

    Please refer to the Certificate of Analysis (CoA).

  • Form

    Lyophilized

  • Formulation

    Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.

    Please contact us for customized product forms or formulations.

  • Reconstitution

    Please refer to the Certificate of Analysis (CoA) for specific instructions.

  • Shipping

    Lyophilized product is shipped at ambient temperature.

  • Storage

    For long term storage, the product should be stored in a lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • Notices

    Product Specific Notices: For research use only.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • Bioactivity-ELISA

     Duocarmycin ELISA

    Serial dilutions of Duocarmycin were added into BSA-Duocarmycin: Monoclonal Anti-Duocarmycin Antibody, Rabbit IgG (M1E06) (Cat. No. DUN-MY2287) binding reactions. The half maximal inhibitory concentration (IC50) is 0.10 μg/mL (QC tested).

    Protocol

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Background

Duocarmycin, a DNA minor groove-binding alkylating agent, acts as a payload in antibody-drug conjugates (ADCs) by selectively targeting AT-rich regions of DNA. It forms covalent bonds with the N3 position of adenine, inducing strand breaks and apoptosis through S-phase cell cycle arrest . This mechanism bypasses multidrug resistance pathways, making it potent against aggressive tumors.

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