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Product Details
Source
Chimeric Monoclonal Anti-Datopotamab Antibody, Mouse IgG1 (7D3) is recombinantly expressed in HEK293. It consists of mouse variable regions genetically fused to mouse IgG1 constant domains.
Antibody Type
Recombinant Monoclonal
Clone
7D3
Isotype
Mouse IgG1, Kappa
Host Species
Mouse
Specificity
7D3 is an inhibitory anti-idiotype monoclonal antibody that specifically recognizes Datopotamab. No detectable binding to the tested human IgG1 controls, Bevacizumab, Cetuximab, or TROP-2 was observed under the tested conditions. 7D3 inhibits the binding of Datopotamab to TROP-2.
Application
ApplicationRecommended Use / PerformanceELISA1-10 ng/mLBlocking Elisa0.11-0.63 μg/mLPK Assay DevelopmentRecommended for use as the capture antibody in a sandwich ELISA for Datopotamab quantification during PK assay development, paired with Biotinylated Anti-Datopotamab Antibody (5D6) as the detection antibody.
(Recommended dilutions are for reference only. Optimal working concentrations may vary depending on your consumables, reagents, and assay conditions. We recommend optimizing for your specific system. No warranty is made regarding performance at any specific dilution.)Purification
Protein A purified.
Aggregation
Less than 5%, as determined by SEC-MALS.
Concentration
Please refer to the Certificate of Analysis (CoA).
Form
Lyophilized
Formulation
Lyophilized from a 0.22 μm-filtered solution in PBS (pH 7.4), with trehalose as protectant.
Please contact us for customized product forms or formulations.
Reconstitution
Please refer to the Certificate of Analysis (CoA) for specific instructions.
Shipping
Lyophilized product is shipped at ambient temperature.
Storage
For long term storage, the product should be stored in a lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Notices
Product Specific Notices: For research use only.
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Performance Data
Bioactivity-ELISA
Datopotamab was quantified using a PK sandwich ELISA with Monoclonal Anti-Datopotamab Antibody, Mouse IgG1 (7D3) (Cat. No. DAB-MY2439a) at 1 μg/mL as the capture antibody and Biotinylated Monoclonal Anti-Datopotamab Antibody, Mouse IgG1 (5D6) (Cat. No. DAB-BLY2438a) at 0.5 μg/mL as the detection antibody. Datopotamab standards were prepared in 10% human serum. Detection was performed using HRP-conjugated streptavidin (QC tested).
Protocol
The specificity of Monoclonal Anti-Datopotamab Antibody, Mouse IgG1 (7D3) (Cat. No. DAB-MY2439a) was evaluated by ELISA. Datopotamab was immobilized at 1 μg/mL (100 μL/well). 7D3 specifically recognized Datopotamab, with no detectable binding to the tested human IgG1 controls, Bevacizumab, Cetuximab, or Human TROP-2 under the tested conditions (Routinely tested).
Protocol
The ability of Monoclonal Anti-Datopotamab Antibody, Mouse IgG1 (7D3) (Cat. No. DAB-MY2439a) to block Datopotamab to TROP-2 was evaluated by ELISA. Datopotamab was immobilized at 1 μg/mL (100 μL/well). Increasing concentrations of 7D3 were tested for their ability to inhibit the biotinylated Human TROP-2 to Datopotamab. 7D3 inhibited Datopotamab binding to TROP-2 in a dose-dependent manner, with an IC₅₀ of approximately 0.26 μg/mL under the tested conditions (Routinely tested).
Protocol
Bioactivity-SPR
The binding affinity of Monoclonal Anti-Datopotamab Antibody, Mouse IgG1 (7D3) (Cat. No. DAB-MY2439a) to Datopotamab was determined by surface plasmon resonance (SPR). Datopotamab was captured on an anti-mouse IgG-coupled CM5 sensor chip, and Datopotamab was injected as the analyte at serial concentrations (0.781-50 nM). The apparent equilibrium dissociation constant (KD) was determined to be 3.40E-09 M (3.4 nM) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
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