Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Research Grade (HEK)Research use only

  • This product is designed for academic and scientific research institutions.
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Cat. No. / Size
Price
Qty
CDE-C15DGN-100ug
$92.00
ETA of in-stock products:2 business days

Product Details

  • Features and Advantages

    1. Native Conformation: Native sequences, tag-free and natural function.
    2. Reliable Activity: Biological activity calibrated against WHO/NIBSC standards.
    3. Stringent Quality Control: Protein content, purity, and cell-based bioactivity testing for each batch.
    4. Lowest Endotoxin level (<0.01 EU/ug).
    5. Safety Assurance: Sterile filtration through 0.2 μm membrane.
    6. AOF: Animal origin-free raw materials throughout the production process.
  • Source

    Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Research Grade (CDE-C15DGN) is recombinantly produced from human 293 cells (HEK293).

  • Isotype

    Mouse IgG2a, Kappa

  • Specificity

    This product is a specific antibody specifically reacts with CD3 epsilon.

  • Endotoxin

    Less than 0.002 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at liquid state at -70°C.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 2-8°C for 12 months under sterile conditions;
    2. -70°C for 24 months.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process
  • Quality Description

    ACRO's Research-grade products are suitable for a wide range of cell culture applications, particularly for research use in academic institutions. These products are sterilized by filtration, followed by lyophilization where applicable. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. Biological activity is calibrated against WHO/NIBSC standards when available.
    ACRO's Premium-grade (Pre-GMP) products are characterized by their high quality and enhanced safety profiles, making them ideal for early-stage discovery and manufacturing processes in cell therapy companies. A key advantage is their seamless transition to corresponding GMP-grade versions. Biological activity is calibrated against WHO/NIBSC standards when available. Typical specifications include endotoxin levels of <0.01 EU/μg and purities >95%. In addition, rigorous testing is conducted to ensure the absence of mycoplasma, HCD, and HCP, thereby guaranteeing product safety.

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Performance Data

  • SDS-PAGE

    CD3 SDS-PAGE

    Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Research Grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-HPLC

    CD3 SEC-HPLC

    The purity of Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Research Grade (Cat. No. CDE-C15DGN) was greater than 95% as determined by SEC-HPLC.

  • Bioactivity-CELL BASE

     CD3 CELL

    Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Research Grade (Cat. No. CDE-C15DGN) stimulates secretion of IL-2 by PBMC stimulated with 10 ng/mL Monoclonal Anti-Human CD28 Antibody, Mouse IgG1. The typical EC50 for this effect is 0.1302 ng/mL (QC tested).

    Protocol

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Background

CD3e molecule, epsilon is also known as CD3E, is a T-cell surface single-pass type I membrane glycoprotein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. CD3E gene has also been linked to a susceptibility to type I diabetes in women. CD3E has been shown to interact with TOP2B, CD3EAP and NCK2.

Recent Advances

 
Drug Development Progress
  • English Name:

    T cell surface glycoprotein CD3

  • Category:

  • Approved Drugs:

    10 Details

  • Drugs in Clinical Trials:

    198 Details

  • Highest Development Stage:

    Approved

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  • Background